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Plasmid#20235DepositorInsertdKDM4A (Kdm4A Fly)
UseTagsFLAG2 and HA2ExpressionInsectMutationchanged His 195 to AlaPromoterAvailable sinceMarch 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
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Neurl1-TOPO
Plasmid#17281DepositorInsertNeuralized-like (Neurl1a Mouse)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
M29 NacMS+LEF>Nac
Plasmid#17179DepositorInsertnacre promoter driving nacre cDNA (mitfa Zebrafish)
UseTagsExpressionMutationAll three Tcf/Lef sites are mutated, but 5 LEF si…PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK E7 del EDE
Plasmid#13675DepositorInsertHPV 16 E7 del EDE (E7 Human)
UseTagsGSTExpressionBacterialMutationdel aa 35-37PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Radical Fringe (CT#475)
Plasmid#13981DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) Radical Fringe (CT#476)
Plasmid#13982DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) flag Radical Fringe (CT#472)
Plasmid#13983DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsFlagExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p031 pCS2 End-1 ORF
Plasmid#11030DepositorInsertEnd-1 (end-1 Nematode)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-Ala11-GFP
Plasmid#126654PurposeInducible expression of Draper-Ala11-GFP mutant in S2 cellsDepositorInsertDraper cytoplasmi Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic Tyr residues to AlaPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-delta ITAM-GFP
Plasmid#126655PurposeInducible expression of Draper-delta ITAM-GFP mutant in S2 cellsDepositorInsertDraper with ITAM Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic ITAM Tyr residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-GFP
Plasmid#126665PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain
UseTagsExpressionInsectMutationK986N- see depositor comments belowPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec633
Plasmid#130873PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationL477V, L640Y, I777K, W814NPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1
Plasmid#17743DepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
myc-Raptor
Plasmid#1859DepositorInsertRaptor (RPTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only