Addgene

We narrowed to 6,547 results for: tev

Showing: 5421 - 5440 of 6547 results

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pcDNA3.1hSIRT3_H248Y_HA

Plasmid

#24492

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

H248Y

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

Plasmid

#130640

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUBQ10:AtFLS2

Plasmid

#202186

Purpose

Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2 (FLS2 Mustard Weed)

Use

Tags

HA

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-DsRed-cNLS

Plasmid

#138533

Purpose

Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-mCherry-cNLS

Plasmid

#138531

Purpose

Lentiviral inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0374 (His-MBP-Cas8_Cas7_Cas6)

Plasmid

#130639

Purpose

Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on Cas8

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-human LRRC33

Plasmid

#111602

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Use

Tags

myc/his tag

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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CAG::Gdnf

Plasmid

#78209

Purpose

piggybac vector for mouse Gdnf expression

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Gdnf (Gdnf Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pTetOne-Six1-myc

Plasmid

#138530

Purpose

Inducible expression of Six1 with a myc tag

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

Six1 (Six1 Mouse)

Use

Tags

3x myc tag

Expression

Mammalian

Mutation

Promoter

TRE3Gs

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-MBP-4xDQNAT

Plasmid

#128398

Purpose

Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Maltose binding protein

Use

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Y342H- please see depositor comments

Promoter

trc

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0376 (His-MBP-StrepII-TniQ)

Plasmid

#130641

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite-StrepII on TniQ

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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tol2-GFAP::CD14

Plasmid

#59593

Purpose

Magnetic cell sorting of ES cell-derived astrocytes

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

human CD14 (CD14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

gfa2

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
33-G-X1-pEF1-puro

Plasmid

#111604

Purpose

Express human 33-G-X1 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2

Plasmid

#96990

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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33-G-X5-pEF1/V5-His

Plasmid

#111605

Purpose

Express human 33-G-X5 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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CNC3 (CASANOVA-C3)

Plasmid

#137191

Purpose

Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3 (CASANOVA-C3)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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