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Addgene

pUBQ10:AtFLS2
(Plasmid #202186)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202186 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGWB14
  • Modifications to backbone
    Modified promoter to UBQ10 promoter
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flagellin sensing 2
  • Alt name
    FLS2
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    FLS2 (a.k.a. AT5G46330, FLAGELLIN-SENSITIVE 2, MPL12.13, MPL12.8, MPL12_13)
  • Promoter AtUBQ10
  • Tag / Fusion Protein
    • HA

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUBQ10:AtFLS2 was a gift from Gitta Coaker (Addgene plasmid # 202186 ; http://n2t.net/addgene:202186 ; RRID:Addgene_202186)
  • For your References section:

    Variation in microbial feature perception in the Rutaceae family with immune receptor conservation in citrus. Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker GL. Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263. 10.1093/plphys/kiad263 PubMed 37144828