pUBQ10:AtFLS2
(Plasmid
#202186)
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PurposeExpress Arabidopsis thaliana FLS2 gene under UBQ10 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB14
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Modifications to backboneModified promoter to UBQ10 promoter
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlagellin sensing 2
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Alt nameFLS2
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SpeciesA. thaliana (mustard weed)
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Entrez GeneFLS2 (a.k.a. AT5G46330, FLAGELLIN-SENSITIVE 2, MPL12.13, MPL12.8, MPL12_13)
- Promoter AtUBQ10
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Tag
/ Fusion Protein
- HA
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUBQ10:AtFLS2 was a gift from Gitta Coaker (Addgene plasmid # 202186 ; http://n2t.net/addgene:202186 ; RRID:Addgene_202186) -
For your References section:
Variation in microbial feature perception in the Rutaceae family with immune receptor conservation in citrus. Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker GL. Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263. 10.1093/plphys/kiad263 PubMed 37144828