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Plasmid#123810PurposeEncodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertBgh pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
NPC1-CFP P692S
Plasmid#58457Purposeexpression of NPC1-CFP sterol sensing domain mutantDepositorInsertNPC1 (Npc1 Mouse)
UseTagsECFP and His6ExpressionMammalianMutationP692S sterol sensing domain mutantPromoterCMVAvailable sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDGE8
Plasmid#79463PurposesgRNA shuttle vector; module 2EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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LentiGuide_CjCas9-Puro
Plasmid#202564PurposehU6-BsmBI-gRNA-PuroDepositorInsertCjCas9 sgRNA cassette
UseTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
fADL-1e-flag-linker-p3
Plasmid#139441PurposeFor cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.DepositorInsertlinker with pelB-leader, cloning site, flag-tag and p3
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sox1/2/3-2373/+3>nls::Cas9::nls
Plasmid#59989PurposeSox1/2/3 (SoxB1) driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Sox1/2/3 (SoxB1) -2373/+3Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-AKT-PH
Plasmid#67674PurposeGateway entry clone containing AKT-PH with no stop codonDepositorInsertAKT-PH
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-H2B-Dendra
Plasmid#140559PurposeRed to green photoconvertible chromosome markerDepositorInsertH2B
UseTagsDendraExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104119PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseAdenoviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGENE:EFF-1::mCherry::V5
Plasmid#133020PurposeEFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cellsDepositorInsertEFF-1::mCherry::V5
UseTagsV5; mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 L232A
Plasmid#8976DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationL232APromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
MTK3_026
Plasmid#123741PurposeEncodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK systemDepositorInsertFlamindo2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NHERF1-PDZ1-2
Plasmid#28294DepositorInsertNHERF1 (NHERF1 Human)
UseTagsFLAGExpressionMammalianMutationContains aa 1-298 (deletion of the EB domain aa 2…PromoterAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pER-eZinCh-2
Plasmid#70009Purposeexpresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cellsDepositorInsertER-eZinCh-2
UseTagsKDEL sequence and PPI signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C-Halo
Plasmid#195153Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE6
Plasmid#79476PurposesgRNA shuttle vector; module 1EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0508
Plasmid#91014PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRecLV103-GFP-PGBD5
Plasmid#65409PurposeLentiviral expression vector for fusion of GFP with human PGBD5DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only