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Plasmid#190798PurposeImaging of synaptopodin in live cellsDepositorInsertSynaptopodin-A (SYNPO Synthetic, Human)
UseTagsCerulean, His, Venus, and mycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorInsertR-GECO1.2
UseTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dsup pLVX-Puro
Plasmid#180635PurposeExpresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio toleranceDepositorInsertDsup
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210A
Plasmid#132965PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 T210A (PLK1 Human)
UseTagsExpressionMammalianMutationT210APromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-ShadowY-kCAAX
Plasmid#115564Purposedark ITRACK acceptor construct for dual color imagingDepositorInsertShadowY with KRas membrane targeting domain
UseTagsShadowYExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
puc19-pCas13a
Plasmid#118963PurposeIntermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.DepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Rac1
Plasmid#83950PurposeRac1 FLIM donor for dynamic imaging of GTPase activityDepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_HP1
Plasmid#82607PurposeExpresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cellsDepositorInsertsfCherry2(11)_HP1b
UseTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCas13 Twin-SUMO
Plasmid#199753PurposeExpresses codon optimized miniature mCas13 for protein expression in E.coliDepositorInsertmCas13
UseTagsSUMOExpressionBacterialMutationPromoterLacIAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-14-3-3 ϵ
Plasmid#48797Purposeexpresses human myc-14-3-3ϵ in mammalian cellsDepositorInsertYWHAE 14-3-3 ϵ (YWHAE Human)
UseTagshaemagglutininExpressionMammalianMutationPromotercmvAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-CAR-GECO1
Plasmid#45493PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
UseTagsExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYX233
Plasmid#163762PurposeEmpty vector for Gal-inducible yeast expressionDepositorTypeEmpty backboneUseTagsHemagglutinin (HA) tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ADRB2-mNG
Plasmid#195145PurposeImage recycling cargoDepositorInsertADRB3 (ADRB3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRT801
Plasmid#68112PurposeIntegration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Hd32
Plasmid#163963PurposeExpresses TPMV H glycoprotein in mammalian cellsDepositorInsertTPMV H glycoprotein
UseTagsExpressionMammalianMutationAmino acids 2 to 33 at N terminus deletedPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-O-GECO1
Plasmid#46025PurposeOrange intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertO-GECO1
UseTagsExpressionMammalianMutationR-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-GCaMP6f
Plasmid#73466PurposeExpresses GCaMP6f for calcium imagingDepositorInsertGCaMP6f
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only