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Plasmid#199550PurposePiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoterDepositorUsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterTRE+CMVmin promoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only
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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113341PurposeExpresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-HA-Blast
Plasmid#175270PurposeMegaGate destination with C-terminal HA tag and blasticidin resistanceDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. aureus scaffold CARGO array
Plasmid#191317PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-HDAC2
Plasmid#222553PurposePiggyBac transposon plasmid for doxycycline inducible expression of HDAC2DepositorInsertHDAC2 (HDAC2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE
Plasmid#223287PurposeA complete OptoMBP cassette for dissolution of FUS-CHOP condensates.DepositorInsertmyc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
UseSynthetic Biology; Piggybac transposase insertabl…TagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_Blasto
Plasmid#167864PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-GFPd2
Plasmid#115665PurposePiggybac transposon plasmid for destabilized eGFP (GFPd2)DepositorInsertGFPd2
UseSynthetic BiologyTagsDestabilizationExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPIDCB2
Plasmid#169900PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.DepositorTypeEmpty backboneUseAvian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceDec. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-DMC1
Plasmid#222541PurposePiggyBac transposon plasmid for doxycycline inducible expression of DMC1DepositorInsertDMC1 (DMC1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1a-MG
Plasmid#175504PurposeAll-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elementsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1aAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-YTHDC2
Plasmid#222558PurposePiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2DepositorInsertYTHDC2 (YTHDC2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-HA-Hygro
Plasmid#175268PurposeMegaGate destination with C-terminal HA tag and hygromycin resistanceDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS
Plasmid#140008PurposePlasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsert4xPylT (Mma) cassette
UseTagsExpressionMammalianMutationU25CPromoterh7SKAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGPF 150TAG
Plasmid#154765Purposeplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. pyogenes scaffold CARGO array
Plasmid#191316PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-BCL2-hygro
Plasmid#222581PurposePiggyBac transposon plasmid for constitutive expression of BCL2DepositorInsertBCL2 (BCL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-DD-Blast
Plasmid#175271PurposeMegaGate destination with C-terminal DD tag and blasticidin resistanceDepositorTypeEmpty backboneUseTagsDestabilization DomainExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCL.140
Plasmid#184995PurposeExpress Cas9 in human cellsDepositorInsertSpCas9
UseTagsSV40NLSExpressionMammalianMutationPromoterTetOn-3gAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only