Addgene

We narrowed to 40,511 results for: Ina;

Showing: 521 - 540 of 40511 results

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

Plasmid

#48285

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159811

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

Frank von Delft, ASAP Discovery

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

C145S mutant

Promoter

Available since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

Plasmid

#122450

Purpose

Expression of inositol 1,4,5-trisphosphate 3-kinase A

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Use

Tags

beta-galactosidase

Expression

Bacterial

Mutation

coding sequence from V66 till stop codon

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

Plasmid

#228635

Purpose

Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

Frank von Delft, ASAP Discovery

Insert

Enterovirus A71 3C protease isoform A

Use

Tags

His-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

Plasmid

#228634

Purpose

Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

Frank von Delft, ASAP Discovery

Insert

Enterovirus Coxsackievirus A71 2A protease

Use

Tags

His-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

Plasmid

#50912

Purpose

Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

Depositor

Biff Forbush

Article

Monette et al J Biol Chem. 2014 Jan 22.

Insert

human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)

Use

Tags

3x Flag and YFP

Expression

Mammalian

Mutation

P676C and I730C; N-term deletion of aa13-222; C-t…

Promoter

CMV

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)

Plasmid

#228661

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assay

Depositor

Frank von Delft, ASAP Discovery

Insert

Zika virus portion of NS2B protease fused to NS3

Use

Tags

His-Twin Strep-Tev

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204797

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

Frank von Delft, ASAP Discovery

Insert

West Nile NS2B-NS3 fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Use

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002-MBP

Plasmid

#102831

Purpose

Bacterial expression of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to the N-terminus of MBP via a …

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003

Plasmid

#133447

Purpose

Expresses SpyCatcher003 in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003

Use

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Use

Tags

His6 and TEV site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher002

Plasmid

#102829

Purpose

Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Cys-SpyCatcher002

Use

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Cysteine residue in N-terminal region

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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SpyTag-sfGFP

Plasmid

#184226

Purpose

Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpyTag-sfGFP

Use

Tags

His6, SpyTag, and TEV protease cleavage site

Expression

Bacterial

Mutation

Promoter

T5

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oDi

Plasmid

#124661

Purpose

Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oDi

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 521 - 540 of 40511 results

Results per page:

Sort results by:

pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)

WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

pDEST14-SpyCatcher002

pET28a-SpyTag002-MBP

SpyCatcher003

SpyTag003-sfGFP

SpyCatcher003-sfGFP

SpyTag003-mKate2

pDEST14-Cys-SpyCatcher002

SpyTag-sfGFP

SpyCatcher002-oDi

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