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We narrowed to 4,181 results for: 027

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pCasper-pNos-MCP-mCherry

Plasmid

#180275

Purpose

to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

MCP-mCherry

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUXB-CA

Plasmid

#125027

Purpose

All-in-one lentivector with tet-inducible DUXB (codon sequences altered)

Depositor

Stephen Tapscott

Article

Chew et al Dev Cell. 2019 Sep 9;50(5):658-671.e7. d

Insert

DUXB (codon altered) (DUXB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1010A

Plasmid

#176078

Purpose

pBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g9608.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010B

Plasmid

#176079

Purpose

pBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g25050.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010C

Plasmid

#176080

Purpose

pBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

Ce FAR-8

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010D

Plasmid

#176081

Purpose

pBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

Ce FAR-8

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-Unstructured-EIF3B

Plasmid

#136027

Purpose

EIF3B 3'UTR with the Artificial Unstructured 3'UTR fused upstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Sema3g.z(S)-AP-His

Plasmid

#72027

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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C0040 (tetR)_CD

Plasmid

#66027

Purpose

MoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Transcription factor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHL1529

Plasmid

#53027

Purpose

PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR
csrA

Use

Synthetic Biology

Tags

RBS(st2), RBS(st3), and glgC leader contains -61 …

Expression

Bacterial

Mutation

Promoter

pConNoHind and pLlac0-1

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only
ELONGIN BC (XX01TCEB1A-c001)

Plasmid

#110274

Purpose

Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

Depositor

Nicola Burgess-Brown

Insert

Elongin BC (ELOC, ELOB Human)

Use

Tags

Expression

Bacterial

Mutation

Elongin B is full length. Elongin C contains a.a…

Promoter

T7

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Tags

Expression

Mutation

See Comments

Promoter

CaMKII

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-RUSH

Plasmid

#202799

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

Lamp1 (Lamp1 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

Plasmid

#133027

Purpose

Expresses EGFP-tagged late endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB7A (RAB7A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

Plasmid

#171027

Purpose

Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1 and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

ChrimsonR is fused to tdTomato

Expression

Mutation

Promoter

Ef1a

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Ad-EphA3 KD

Plasmid

#102726

Purpose

Expression of Kinase dead EphA3

Depositor

Jin Chen

Article

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Tags

Expression

Mutation

Kinase Dead (K657M; AAA > ATG)

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Tfap2a

Plasmid

#70274

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Tfap2a (Tfap2a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pGV3341

Plasmid

#170279

Purpose

Expression of mutant HIV-2 RT p66 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p66 (mutant)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7-lac

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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