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pBbdCas9S(-10TC)_Psyn-sgRNArodA
Plasmid#149657Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAflaB
Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM65905
Plasmid#153217PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformationDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m2
Plasmid#84721PurposepVR2 but with a G to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at position 16 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m3
Plasmid#84722PurposepVR2 but with a C to G mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationC to G mutation at position 22 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR
Plasmid#84718PurposeE. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilisDepositorInsertsE. coli RecA promoter
5' UTR of queC gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m1
Plasmid#84720PurposepVR2 but with a C to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationC to U mutation at position 13 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG SMAD4
Plasmid#83094PurposeGateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tagDepositorInsertSMAD4 (SMAD4 Human)
UseEntry vector for gateway cloningTags3xFLAGExpressionMutationPromoterTRE tight promoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5-V5 blast
Plasmid#83100PurposeLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTGC-U6.3
Plasmid#112810PurposePCR template vector for amplifying gRNAcore-pU6.2 promoter fragmentDepositorInsertU6:3-gRNAcore
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-PdhR
Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mus-Bnip3_deltaExon3
Plasmid#100793PurposeMammalian expression of Bnip3_deltaExon3 splice variantDepositorInsertmouse-Bnip3_deltaExon3 (Bnip3 Mouse)
UseTagsHAExpressionMammalianMutationExon3 deletionPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only
