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Showing: 5261 - 5280 of 51114 results

  6. pAAV-CMV-dSa VP64 Afp

    Plasmid
    #99697
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    99697-plasmid-map-sequence-id-194575

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  7. pRS416 CUP1pro-Htt103QdeltaP125-134-YFP

    Plasmid
    #224882
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP125-134 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP125-134
    Promoter
    CUP1
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224882-plasmid-map-sequence-id-448243

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  8. pRS416 CUP1pro-Htt103QdeltaP135-170-YFP

    Plasmid
    #224878
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP135-170 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP135-170
    Promoter
    CUP1
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224878-plasmid-map-sequence-id-448235

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  9. pRS416 CUP1pro-Htt103QdeltaP141-160-YFP

    Plasmid
    #224886
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP141-160 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP141-160
    Promoter
    CUP1
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224886-plasmid-map-sequence-id-448229

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  10. pRS416 CUP1pro-Htt103QdeltaP125-160-YFP

    Plasmid
    #224883
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP125-160 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP125-160
    Promoter
    CUP1
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224883-plasmid-map-sequence-id-448180

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  11. pRS416 CUP1pro-Htt103QdeltaP141-170-YFP

    Plasmid
    #224880
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP141-170 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP141-170
    Promoter
    CUP1
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    224880-plasmid-map-sequence-id-448151

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  12. pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

    Plasmid
    #224876
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    HTT103QdeltaP161-170 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    delta161-170
    Promoter
    CUP1
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    224876-plasmid-map-sequence-id-448047

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  13. SOX2-3'gRNA

    Plasmid
    #215543
    Purpose
    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in
    Depositor
    Kai Wang
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215543-plasmid-map-sequence-id-424976

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  14. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Kai Wang
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    215544-plasmid-map-sequence-id-424979

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  15. pGU06

    Plasmid
    #212148
    Purpose
    Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Agx1: S. cerevisiae; alaA, tyrB: E. coli, Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212148-plasmid-map-sequence-id-420636

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  17. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid
    #199781
    Purpose
    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).
    Depositor
    Sascha Martens
    Insert
    NEMO (IKBKG Human)
    Use
    Tags
    GST-Thrombin-TEV-EGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199781-plasmid-map-sequence-id-418258

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  18. pYCTK010

    Plasmid
    #176714
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    mfalpha1Ka
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176714-plasmid-map-sequence-id-351596

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  19. pYCTK006

    Plasmid
    #176710
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter SST2 (response part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-SST2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176710-plasmid-map-sequence-id-351557

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  20. pYCTK001

    Plasmid
    #176705
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter DIG2 of S. cerevisiae (response part)
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-DIG2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    176705-plasmid-map-sequence-id-351600

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Showing: 5261 - 5280 of 51114 results