Addgene

We narrowed to 6,280 results for: aav

Showing: 5221 - 5240 of 6280 results

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TetO::EGFP-C-Meikin

Plasmid

#174706

Purpose

Doxycycline inducible expression of human C-Meikin Separase cleavage fragment

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Tags

EGFP

Expression

Mammalian

Mutation

155-373

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRC_RR_VP2/3

Plasmid

#65725

Purpose

wt VP2/VP3 only to participate in capsid assembly; no ITR's

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

empty

Use

Tags

Expression

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDonor MCS Rosa26

Plasmid

#37200

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Inserts

Rosa26 left targeting arm
MCS
Rosa26 right targeting arm

Use

Genomic targeting - zinc finger

Tags

Expression

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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CLAPon

Plasmid

#213405

Purpose

Peroxidase (APEX) reporter for TEV protease cleavage

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMK382

Plasmid

#214373

Purpose

Tet-OsTIR1(F74G) (AAVS1 insertion donor)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

Tet-OsTIR1(F74G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet ON 3G promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMVS184

Plasmid

#176294

Purpose

Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Insert

Promoter and GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

4x Zinc finger binding sites and mini CMV promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pVP2del50

Plasmid

#125185

Purpose

Expresses the VP2del50 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Use

Tags

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2697

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pVP2del30

Plasmid

#125184

Purpose

Expresses the VP2del30 truncation mutant protein derived from AAV2 VP2

Depositor

Junghae Suh

Article

Thadani et al ACS Nano. 2018 Feb 27;12(2):1445-1454. d

Insert

hexahistidine tag

Use

Tags

Expression

Mammalian

Mutation

Inserted start codon and hexahistidine tag at 2637

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12i1

Plasmid

#120882

Purpose

Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c1

Plasmid

#120872

Purpose

Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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AX53_pS.Donor.S1.TS

Plasmid

#100290

Purpose

AAVS1-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

Plasmid

#104119

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Mutation

Promoter

EF1-Alpha

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-ReaChR-citrine

Plasmid

#50956

Purpose

ReaChR-citrine in lentival transfer vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

Lentiviral

Tags

citrine

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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H52-pUCM-hNGN2-inv

Plasmid

#110492

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

Depositor

Michael Ward

Inserts

Neurogenin 2 (NEUROG2 Human)
mcherry
rtTA3G

Use

CRISPR, Cre/Lox, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG, EF-1 alpha, and TRE3G

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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