Addgene

We narrowed to 762 results for: xenopus

Showing: 501 - 520 of 762 results

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Jennifer Tirnauer, Tim Mitchison

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pCS_FLPER(T2)

Plasmid

#31303

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

full length FLP linked to ligand binding domain o…

Promoter

Availability

Academic Institutions and Nonprofits only

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pSGEMKv10.1

Plasmid

#85704

Purpose

Xenopus laevis oocyte expression of Kv10.1

Depositor

Luis Pardo

Article

Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

Insert

KCNH1 (KCNH1 Human)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Zebrafish lim5 (lhx5)

Plasmid

#74141

Purpose

Basic cDNA clone for zebrafish lim5 (lhx5). Cloned in BS KS+.

Depositor

Igor Dawid

Article

Toyama et al Dev Biol. 1995 Aug;170(2):583-93.

Insert

lhx5 (lhx5 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Inserts

TVA (CD320 Chicken)
TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

sCMV and sCMV IE94

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

trc

Availability

Academic Institutions and Nonprofits only

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pCS2 mt-GFP (BamHI minus)

Plasmid

#15681

Depositor

Michael Klymkowsky

Type

Empty backbone

Use

Xenopus/mammalian/avian/zebrafish

Tags

6x Myc and GFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Use

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Promoter

Availability

Academic Institutions and Nonprofits only
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Promoter

Availability

Academic Institutions and Nonprofits only
M34 Xdsh PDZ bait-pGBT9

Plasmid

#17190

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Xdsh (dvl2.L Frog)

Use

Tags

Gal4-DBD

Expression

Yeast

Mutation

Dsh bp 343-1191

Promoter

Availability

Academic Institutions and Nonprofits only
M35 Dpr yeast clone

Plasmid

#17191

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr (dact1-b Frog)

Use

Tags

Gal4-AD

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
M36 Dpr C-terminal GST

Plasmid

#17192

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr (dact1-b Frog)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
HA GSK3 beta wt pcDNA3

Plasmid

#14753

Purpose

Mammalian expression of human GSK3 beta with HA tag

Depositor

Jim Woodgett

Article

He et al Nature. 1995 Apr 13. 374(6523):617-22.

Insert

GSK3 beta (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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mOtop2_pGEMHE

Plasmid

#114675

Purpose

This construct was used to express mouse Otop2 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Use

For generating the mrna, which can highly express…

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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mOtop3_pGEMHE

Plasmid

#114676

Purpose

This construct was used to express mouse Otop3 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

For generating the mrna, which can highly express…

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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GST-peripherin-CTS

Plasmid

#86070

Purpose

A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Peripherin (prph2.S Frog)

Use

Tags

GST

Expression

Bacterial

Mutation

contains the region from 317-336 of peripherin

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Tags

Expression

Mutation

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:LifeAct-mRuby)

Plasmid

#166977

Purpose

labels F-actin in macrophages

Depositor

Anna Huttenlocher

Article

Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

Inserts

mpeg1 (mpeg1.1 Zebrafish)
LifeAct (actb1 Frog)

Use

Tags

Lifeact-mRuby and mRuby

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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