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Addgene

pCS_FLPER(T)
(Plasmid #31131)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31131 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLP-ER ligand binding domain
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2300
  • Mutation
    full length FLP linked to ligand binding domain of estrogen receptor starting at AA 251. Contains V400G and G521R.
  • Entrez Gene
    ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tamoxifene regulated FLP activity

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS_FLPER(T) was a gift from Gerhart Ryffel (Addgene plasmid # 31131 ; http://n2t.net/addgene:31131 ; RRID:Addgene_31131)
  • For your References section:

    FLP and Cre recombinase function in Xenopus embryos. Werdien D, Peiler G, Ryffel GU. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3. 10.1093/nar/29.11.e53 PubMed 11376165