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Plasmid#120763PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 3 (P3_EYK300-4AB)DepositorInsertP3_EYK300-4AB
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE273
Plasmid#120762PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 3 (P3_EYK300-3AB)DepositorInsertP3_EYK300-3AB
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE292
Plasmid#120759PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_2UASxpr-TEF)DepositorInsertP3_2UASxpr-TEF
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK010
Plasmid#128227Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 4DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 4
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK011
Plasmid#128228Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 5DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 5
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK012
Plasmid#128229Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 6DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 6
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH87
Plasmid#128200PurposesgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH89
Plasmid#128202PurposecrRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH90
Plasmid#128203PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH103
Plasmid#128208PurposecrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH104
Plasmid#128209PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-S. tuberosum trnV/3'rps12
Plasmid#123585PurposeMoChlo kitDepositorInsertS. tuberosum trnV/3'rps12 integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum trnV/3'rps12
Plasmid#123581PurposeMoChlo kitDepositorInsertN. tabacum trnV/3'rps12 integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-rps2 5'UTR mCCUC (-20 to -10 deletion)
Plasmid#123536PurposeMoChlo kitDepositorInsertrps2 5'UTR mCCUC (-20 to -10 deletion)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-rps2 5'UTR mAAGA (-20 to -10 deletion)
Plasmid#123535PurposeMoChlo kitDepositorInsertrps2 5'UTR mAAGA (-20 to -10 deletion)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-atpB 5'UTR TCR processed -90 (functional)
Plasmid#123523PurposeMoChlo kitDepositorInsertatpB 5'UTR TCR processed -90 (functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-atpB 5'UTR +10 processed -90 (functional)
Plasmid#123522PurposeMoChlo kitDepositorInsertatpB 5'UTR +10 processed -90 (functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet50K
Plasmid#118399Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMvuTFIIB mini intein
UseTagsExpressionBacterialMutationincorporation of oligonucleotide during cloningPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC S431A T432A NS1
Plasmid#101837Purposedrives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431A T432A with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431A T432APromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC S431E T432E NS1
Plasmid#101836Purposedrives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431E T432E with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431E T432EPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only