Addgene

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pTT3 Unc5B-FLAG

Plasmid

#72195

Purpose

Expresses full-length Unc5b with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5b (Unc5b Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/GD-IRES-GFP-CTNNB1

Plasmid

#192868

Purpose

Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

Depositor

David Largaespada

Article

Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

Inserts

luc+
EGFP
CTNNB1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119067

Purpose

Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119065

Purpose

Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5C-FLAG

Plasmid

#72196

Purpose

Expresses full-length Unc5c with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c (Unc5c Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Ai32

Plasmid

#34880

Purpose

Cre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitation

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

Floxed ChR2(H134R)-EYFP

Use

Mouse Targeting

Tags

EYFP

Expression

Mutation

H134R in ChR2

Promoter

CAG

Available since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pSI-check2-CUL1-3'UTR

Plasmid

#97156

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

CUL1 3'UTR (CUL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 Zscan4 promoter

Plasmid

#69249

Purpose

Luciferase reporter for Dux4 activation of Zscan4 transcription

Depositor

Stephen Tapscott

Article

Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

Insert

Zscan4 (ZSCAN4 Human)

Use

Luciferase

Tags

Expression

Mutation

The two single nucleotide mismatches found betwee…

Promoter

Zscan4

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Clathrin light chain

Plasmid

#83032

Purpose

Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 508)

Plasmid

#195030

Purpose

GAL4-Creb5 fusion construct (full length, 508aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5A-FLAG

Plasmid

#72194

Purpose

Expresses full-length Unc5a with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a (Unc5a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa120-433

Plasmid

#73411

Purpose

Expresses HA tagged aa120-433 AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 120-433 of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
p7SL-neo-TET

Plasmid

#51285

Purpose

expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

7SL RNA (RN7SL2 Human)

Use

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Mutation

Promoter

7SL upstream pollII enhancer

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLNCX2-STIM1

Plasmid

#89817

Purpose

Express HUMAN STIM1

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

NO TAG

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pIMVAu1

Plasmid

#98301

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-408

Plasmid

#73412

Purpose

Expresses HA tagged aa1-408 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-408 of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pX330-NL-DHFR-SpCas9

Plasmid

#124522

Purpose

Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NL-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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