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Plasmid#105855PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG1 with five mutat…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_CH2charge
Plasmid#105852PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutations modify charge of CH2 domain.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG1, muatations: Gl…PromoterhEF1-HTLV promAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AL62_pDonor.DMD.TS
Plasmid#100287PurposeDMD intron 43-targeting plasmidDepositorInsertEGFP expression unit (DMD Human, Synthetic)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161Q, K163Q
Plasmid#78792PurposeRetroviral expression of p21 K161Q, K163QDepositorInsertP21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161Q, K163Q muationPromoter5'LTRAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS3-MT-BX-6MYC p21 90-164
Plasmid#78786PurposeTo overexpress p21 90-164 in Mammalian CellDepositorInsertP21 (CDKN1A Human)
UseTags6xMYCExpressionMammalianMutationaa90-164PromoterCMV IE94Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS3-MT-BX-6MYC p21 90-139
Plasmid#78785PurposeTo overexpress p21 90-139 in Mammalian CellsDepositorInsertp21 (CDKN1A Human)
UseTags6xMYCExpressionMammalianMutationcontains p21 amino acids 89-139PromoterCMV IE94Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161R, K163R
Plasmid#78791PurposeRetroviral expression of p21 K161R, K163RDepositorInsertp21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161R, K163R_muationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Keratin-17
Plasmid#56164PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659DepositorInsertKeratin (KRT18 Human)
UseTagsmCardinalExpressionMammalianMutationV282LPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC
Plasmid#69585PurposeExpresses mutated p53-L344A tagged with mKate2 and split C-term mVenusDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
UseTags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7b
Plasmid#104484PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7b
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNK3071
Plasmid#219755PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.DepositorInsertmcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7s
Plasmid#104463PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7s
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-mCherry-TOSI
Plasmid#172496PurposemCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.DepositorInsertPDCD4 (Pdcd4 Mouse)
UseRetroviralTagsMcherryExpressionMammalianMutationPromoterMLV LTRAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mAC-POLR2A donor (Hygro)
Plasmid#124496PurposeA donor plasmid for tagging the N-terminus of human POLR2A with mAID-mCloverDepositorInsertPOLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
UseCRISPRTagsmAID-mCloverExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVTagsExpressionMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only