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Addgene

pPBbsr2-Gnaq
(Plasmid #170298)

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Item Catalog # Description Quantity Price (USD)
Plasmid 170298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A recombinant mouse Gnaq
  • Alt name
    Gnaq
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Mutation
    Asilent mutation was introduced into the sgRNA-targeted region
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
  • Tag / Fusion Protein
    • N/A

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBbsr2-Gnaq was a gift from Michiyuki Matsuda (Addgene plasmid # 170298 ; http://n2t.net/addgene:170298 ; RRID:Addgene_170298)

  • For your References section:

    Intravital imaging identifies the VEGF-TXA2 axis as a critical promoter of PGE2 secretion from tumor cells and immune evasion. Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M. Cancer Res. 2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 10.1158/0008-5472.CAN-20-4245. 10.1158/0008-5472.CAN-20-4245 PubMed 34035084
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