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Showing: 5081 - 5100 of 6971 results

  1. P[acman]-Omega1

    Plasmid
    #165860
    Purpose
    Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165860-plasmid-map-sequence-id-336787

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  8. PB-TAC-Cas9

    Plasmid
    #120352
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120352-plasmid-map-sequence-id-237238

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  9. PB-TAC-KRAB-dCas9

    Plasmid
    #120355
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120355-plasmid-map-sequence-id-237262

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  11. IBMc050

    Plasmid
    #161938
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plux2
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161938-plasmid-map-sequence-id-327548

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  13. pSWD95D293A

    Plasmid
    #159099
    Purpose
    CckA FRET Sensor with DITE motif mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CckA D293 mutated to A293 and V199I on mRuby3 (P…
    Promoter
    Xylose
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159099-plasmid-map-sequence-id-316589

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  14. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Tanja Kortemme
    Article
    Thompson et al Elife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    134818-plasmid-map-sequence-id-313944

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  17. C335

    Plasmid
    #141125
    Purpose
    Expresses chimeric repressor (RbsR-L) under Cin promoter
    Depositor
    Matthew Bennett
    Article
    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:
    Inserts
    LLhR
    AiiA (AQ980_RS12585 Synthetic)
    Use
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Chimera: DNA binding domain of LacI and ligand bi…
    Promoter
    engineered cin promoter (responds to C14-HSL) and…
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141125-plasmid-map-sequence-id-298364

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  18. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al Elife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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Showing: 5081 - 5100 of 6971 results