We narrowed to 60,201 results for: %s

1. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

   Plasmid

   #172105

   Purpose

   Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
   

   Expression

   Mammalian

   Mutation

   Gαs: C3S

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDAX-EMMI-027 mCherry(S-T)//Esp3I

   Plasmid

   #130229

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

   Plasmid

   #72611

   Purpose

   For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   changed serine33, threonine34 and serine37 to ala…

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pF(UG) hSyn NS34a-msGFP-'S-mito

   Plasmid

   #158768

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Mutation

   Flexible linkers between the protease and msGFP a…

   Availability

   Academic Institutions and Nonprofits only

   

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5. EasyClone-MarkerFree Vector Set

   Plasmid Kit

   #1000000098

   Purpose

   Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

   Depositor

   Irina Borodina

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. MoClo Yeast Toolkit

   Plasmid Kit

   #1000000061

   Purpose

   96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes

   Depositor

   John Dueber

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204340

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-s/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

   Plasmid

   #164845

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Tags

   8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression. Has N5…

   Promoter

   CMV

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204342

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]

    Plasmid

    #133329

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All FYW to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

    Plasmid

    #133328

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry

    Plasmid

    #133322

    Purpose

    Bacterial expression of TDP43 with FYW-S mutant CTD

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Bacterial

    Mutation

    All FYW in the TDP43 IDRs mutated to S

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry

    Plasmid

    #133321

    Purpose

    Bacterial expression of TDP43 with F-S mutant CTD

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Bacterial

    Mutation

    All F in the TDP43 IDRs mutated to S

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

    Plasmid

    #133333

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All W to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

    Plasmid

    #105182

    Purpose

    Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(E2F1)p-ECFP (Cerulean) sponge control
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. E1b-GFP-Tol2-Gateway dre SE-zic2a S

    Plasmid

    #89388

    Purpose

    Vector for reporter assay of one zebrafish super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    zic2a (zic2a Zebrafish)
    

    Available Since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

18. JO-pH720-SARS-CoV-2-S-tail-WT

    Plasmid

    #218453

    Purpose

    Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
    

    Tags

    eXact

    Expression

    Bacterial

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT

    Plasmid

    #218460

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues)-wild type (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

    Plasmid

    #218461

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
    

    Expression

    Mammalian

    Mutation

    Thr1273AGlu substitution

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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