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pmScarlet3_C1

Plasmid

#189753

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMVHA E2F1

Plasmid

#24225

Purpose

Mammalian expression of human E2F1 with HA tag

Depositor

Kristian Helin

Publication

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F1 (E2F1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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FUW-SOKM

Plasmid

#20325

Purpose

Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Publication

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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PIK3CA-WT

Plasmid

#16643

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIK3CA (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Publication

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Publication

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

99 Articles

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Publication

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Publication

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pET His6 GST TEV LIC cloning vector (1G)

Plasmid

#29655

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Publication

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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HDAC7 Flag

Plasmid

#13824

Purpose

Mammalian expression of human histone deacetylase 7 with flag tag

Depositor

Eric Verdin

Publication

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC7 (HDAC7 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Publication

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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EF.STAT3DN.Ubc.GFP

Plasmid

#24984

Purpose

Depositor

Linzhao Cheng

Publication

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Y705F--dominant negative

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET21a-BirA

Plasmid

#20857

Depositor

Alice Ting

Publication

Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

BirA

Tags

6xHis

Expression

Bacterial

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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