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Plasmid#118370PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.DepositorInsertactin beta (ACTB Human)
UseTagsHA-GFP1-10ExpressionMammalianMutationPromoterCMV/TOAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-EGFP
Plasmid#127591PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Sec61β
Plasmid#174466PurposeExpress EGFP with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertSirius with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP-G3BP2(2)
Plasmid#136061PurposeG3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)DepositorInsertG3BP2 (G3BP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-CHS3-msGFPx3
Plasmid#105257PurposePlasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.DepositorInsertChs3 portion (CHS3 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd MafA-I-nGFP
Plasmid#19412DepositorInsertv-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)
UseAdenoviral; Entry vectorTagsGFP and IRESExpressionMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hG9a-ΔSET
Plasmid#33026DepositorInserthG9a
UseTagsEGFPExpressionMammalianMutationDeletion of the SET domain; contains aa 1-758PromoterCMVAvailable sinceApril 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A-2A-GFP
Plasmid#118270PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
UseTagsC terminal TEV GFP octa-His affinity tagExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQC-mCherryFP-GFP
Plasmid#129102PurposemCherryFP positive controlDepositorInsertmCherryFP
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-EGFP-CA
Plasmid#13886DepositorInsertCA domains of N-WASP (WASL Bovine)
UseAdenoviralTagsEGFPExpressionMutationPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE-GFP-NMHC II-A
Plasmid#10844DepositorInsertNMHC II-A (MYH9 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-GFP
Plasmid#128065PurposeAdenoviral shuttle vector with CMV promoter-EGFPDepositorInsertCMV promoter-EGFP
UseAdenoviralTagsExpressionMutationnoPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL-pEGFP-C1
Plasmid#166735PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BimELDepositorInsertBimEL (Bcl2l11 Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATF4 6: mCD36.GFP
Plasmid#21853DepositorInsertmCD36 (Cd36 Mouse)
UseTagsEGFPExpressionMammalianMutationOnly the first 13 AA of CD36 are fused to GFPPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRE-GFP-NMHC II-B
Plasmid#10845DepositorInsertNMHC II-B (MYH10 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shGFP
Plasmid#110419PurposeshGFP control, silence GFP gene, blasticidin selection.DepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP
Plasmid#138657Purposestable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yeGFP
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Ncad-GFP
Plasmid#35986DepositorInsertNcad (cdh2 Zebrafish)
UseGal4 inducibleTagsEGFPExpressionMutationPromoter14xUAS-E1bAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only