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Plasmid#120462PurposeBarcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pEGFP-C1
Plasmid#71378PurposeFor analysis of PH domain localization in mammalian cellsDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsEGFPExpressionMammalianMutationPleckstrin homology (PH) domain; aa 247-399PromoterCMVAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCD86-EGFP
Plasmid#133858PurposeMammalian expression of CD86 C-terminally tagged with EGFPDepositorInsertCD86 (CD86 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRV∆L-5Cre
Plasmid#182964PurposeThe 3rd generation of rabies vector expressing Cre recombinaseDepositorInsertCRE recombinase
UseRabiesTagsExpressionMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-DDX4LCD
Plasmid#204408PurposeE. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tagDepositorInsertDDX4(1-236) (DDX4 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-4082NES(ForAMPK)
Plasmid#170310PurposeThe lentiviral vector for AMPK FRET-biosensorDepositorInsert4082NES (FRET biosensor for AMPK activity)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_743
Plasmid#176662PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG4K5
Plasmid#74492PurposeFor cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YN
Plasmid#74721PurposeExpress C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YC
Plasmid#74722PurposeExpress C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsA
Plasmid#107558PurposePlasmid for titrating synthesis of FtsA with doxycyclineDepositorInsertftsA
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-LexGAD-3xP3-RFP
Plasmid#127558PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertLexGAD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRRP12_K
Plasmid#146766PurposeMammalian Expression of HsRRP12DepositorInsertHsRRP12 (RRP12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPIB_P2A_Hygro_Barcode
Plasmid#120483PurposeBarcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSPIB (SPIB Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-μ1A-(HA)3
Plasmid#198177PurposeExpression of HA-tagged AP-1 μ1A in mammalian cellsDepositorInsertAP-1 μ1A
UseTagstriple HA tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBT-PE2-PGK-Blast
Plasmid#173219PurposepiggyBac transposon containing PE2 and Blasticidin expression cassettesDepositorInsertsPE2
Blast
UsePiggybacTagsExpressionMammalianMutationPromoterCMV and hPGKAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab6 T27N
Plasmid#46782Purposeexpression of T27N Rab6a in mammalian cellsDepositorInsertRab6 T27N (RAB6A Human)
UseTagsmycExpressionMammalianMutationT27N dominant negativePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
UseTags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only