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Plasmid#118864Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFPDepositorInsertNcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, EGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 short UTR
Plasmid#21648DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
TR-5'UTR-HP-GFP
Plasmid#80594PurposeExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorInsertERGIC2-WT (ERGIC2 Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D500-630
Plasmid#19841DepositorInsertMKL1 D500-630 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 500-630PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW2
Plasmid#166708Purposeexpresses NEDD4L with inactivating mutation in second WW domain in mammalian cellsDepositorInsertNEDD4L W453A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 453 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW3
Plasmid#166709Purposeexpresses NEDD4L with inactivating mutation in third WW domain in mammalian cellsDepositorInsertNEDD4L W525A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 525 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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gamma2 CR shRNA (shRNA2)
Plasmid#120794PurposeshRNA targeting the coding region of the gamma2 mRNADepositorInsertGABRG2 shRNA (Gabrg2 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIS1 Cyclin D2 long-mut-miR15/16
Plasmid#21647DepositorInsertCyclin D2 long-mut-miR15/16 (CCND2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant miR15/16 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
M47 MBP-TEV-Chibby
Plasmid#17195DepositorInsertChibby (CBY1 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35-mut
Plasmid#87341PurposeTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP35 (NUP35 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 humanN-Flag SadBS
Plasmid#66881PurposeMammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadBDepositorInsertSadB (BRSK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_deltaOB
Plasmid#166408Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertPOT1 ΔOB (POT1 Human)
UseTagsmycExpressionMammalianMutationΔOBPromoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_IRES_hygro
Plasmid#166406Purposeexpress hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup88-mut
Plasmid#87337PurposeTo express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP88 (NUP88 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only