Addgene

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p5E_(-3kb)nrg1 type II

Plasmid

#199393

Purpose

pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

(-3kb)nrg1 type II

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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p3E_afp_UTR

Plasmid

#199341

Purpose

pAMS448; Gateway 3' entry clone encoding ocean pout antifreeze (afp) protein 3'UTR

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

afp 3' UTR

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_ngn1_LSE_C

Plasmid

#199395

Purpose

pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

LSE_C

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Violet

Plasmid

#183139

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Violet

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Violet

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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C122-E15: mOrange

Plasmid

#162895

Purpose

Standard Gateway entry clone encoding mOrange fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Orange fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E14: mKate

Plasmid

#162894

Purpose

Standard Gateway entry clone encoding mKate fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E10: mCherry

Plasmid

#162892

Purpose

Standard Gateway entry clone encoding mCherry fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E06: gLuc

Plasmid

#162890

Purpose

Standard Gateway entry clone encoding Gaussia luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Gaussia princeps luciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E01: rLuc

Plasmid

#162888

Purpose

Standard Gateway entry clone encoding Renilla luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Renilla luciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E54: mRuby3

Plasmid

#162899

Purpose

Standard Gateway entry clone encoding mRuby3 fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMM0494

Plasmid

#128981

Purpose

LEU2 preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

LEU2 5' homology-ConLS-sfGFP dropout-ConR1-LEU2 3' homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG131

Plasmid

#105337

Purpose

Gateway entry clone generation

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PlLacO1

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Use

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Use

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Promoter

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-gRNA-EF1a-EGFP-CAAX

Plasmid

#167936

Purpose

gRNA entry vector

Depositor

Emma Rawlins

Article

Sun et al Elife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

U6-gRNA backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pME-ERT2-Cre-ERT2

Plasmid

#82587

Purpose

Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

ERT2-CRE-ERT2

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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EEF1A2 in pENTR

Plasmid

#216585

Purpose

gateway cloning donor vector containing EEF1A2

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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