YCe1491 HC_Kan_RFP-p19
(Plasmid
#100629)
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Purpose(Empty Backbone) EMMA toolkit part-entry vector position 19
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMART HC_Kan
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 19 of EMMA platform.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCe1491 HC_Kan_RFP-p19 was a gift from Yizhi Cai (Addgene plasmid # 100629 ; http://n2t.net/addgene:100629 ; RRID:Addgene_100629) -
For your References section:
EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644