Addgene

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pPaxillin-Electra2

Plasmid

#184926

Purpose

Expresses paxillin fused to blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

paxillin-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4-UPRE-luc2P-Hygro

Plasmid

#101788

Purpose

Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xUPRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

minimal TATA-box promoter with low basal activity

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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LYN11-FRB-CFP

Plasmid

#38003

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

LYN (LYN Human)
MTOR (MTOR )

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
HcKCR2_mCherry_pcDNA3.1

Plasmid

#177337

Purpose

Expresses KCR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR2

Use

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-mRuby2-Gephyrin.FingR-IL2RGTC

Plasmid

#125696

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mRuby2-Gephyrin.FingR-IL2RGTC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-152

Plasmid

#118566

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH623

Plasmid

#171633

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-non-targeting
mNeon

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and U6

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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ePPEplus

Plasmid

#205241

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Tags

Expression

Plant

Mutation

Detailed in manuscript

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-135

Plasmid

#118521

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra2

Plasmid

#179479

Purpose

Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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35S-GWY-VP16/pAM

Plasmid

#201232

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-VP16

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-130

Plasmid

#118516

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-135

Plasmid

#118553

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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