Addgene

We narrowed to 5,521 results for: #16

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pEGFP-mSmo

Plasmid

#25395

Depositor

Philip Beachy

Article

Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

Insert

mouse Smoothened cDNA (Smo Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pdCas9- AsiA_m2.1

Plasmid

#158065

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m2.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-GFP

Plasmid

#87662

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPL5071_TEF1*-Cre_URA3

Plasmid

#60930

Purpose

Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179027

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP670

Plasmid

#45462

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMM112

Plasmid

#127229

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LV-Cre pLKO.1

Plasmid

#25997

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

Cre recombinase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pZ8-T_dCas9

Plasmid

#74062

Purpose

pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pXCX.Syn.EGFP.WPRE

Plasmid

#38052

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin (human SYN1)

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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Vector 1174D

Plasmid

#200012

Purpose

Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

Insert

Engineered dobulesex splicing module

Use

Synthetic Biology

Tags

Expression

Insect

Mutation

Engineered dobulesex splicing module

Promoter

Hr5IE1

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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H2B-SNAPf-mTurquoise2

Plasmid

#197494

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_D4910

Plasmid

#127220

Purpose

Module D with ZmUbi1::BBM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

BBM

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_HaloTag9

Plasmid

#169324

Purpose

Expression of HaloTag9 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

His

Expression

Bacterial

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDB016

Plasmid

#99220

Purpose

Accessory plasmid for aaRS PACE; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII-neg

Use

Tags

Expression

Bacterial

Mutation

Promoter

PT7

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCruzHA SIRT1

Plasmid

#10962

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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p18S.1 wt

Plasmid

#51727

Purpose

transcribes wild type 18S without modifications

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Use

Tags

Expression

Mutation

Promoter

pol-I

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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