Addgene

We narrowed to 9,763 results for: #16

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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FLAG-EglN2 H358A-pBabe-puro

Plasmid

#22705

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated cDNA: point mutation in codon 358 changi…

Promoter

Available since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV40-EF1a-hBCL10-SO

Plasmid

#75256

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs, EF1a

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc1/bC - AVITEV

Plasmid

#74059

Purpose

retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75244

Purpose

CRISPR/Cas9 plasmid against human Helios

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLL5.0 mCherry_K-RasC14

Plasmid

#101286

Purpose

Lentiviral expression of K-Ras C14

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

K-Ras (KRAS Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

C14

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV40-EF1a-hBCL10(1-114)-SO

Plasmid

#75257

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

AS 1-116 (CARD-domain)

Promoter

pMSCV-LTRs, EF1a

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-Keratin-17

Plasmid

#56164

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Keratin (KRT18 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
BAP1-C91A (siRNA resistant)

Plasmid

#108438

Purpose

Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

C91A: c.271T>G, c.272G>C; others: c.1641C&g…

Promoter

CMV

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H4

Plasmid

#42633

Depositor

Joe Landry

Insert

Human H4 (H4C1 Human)

Use

Tags

Untagged

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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RGS16

Plasmid

#39140

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RGS16 (RGS16 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
RG6

Plasmid

#80167

Purpose

fluorescent protein splicing reporter

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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BTV

Plasmid

#84771

Purpose

bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production

Depositor

David Erle

Article

Zhao et al Nat Biotechnol. 2014 Apr;32(4):387-91. d

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-mito

Plasmid

#168304

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-mito

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-NES

Plasmid

#168301

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD33-Gm

Plasmid

#65098

Purpose

bacterial expression vector with Gentamycin selection, based on pBAD33

Depositor

Juan Tomas

Article

Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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