Addgene

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Showing: 4721 - 4740 of 9139 results

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pPTK005-3a-αMF

Plasmid

#84964

Purpose

Type 3a, α-mating factor - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-2a-6MTnlstdTomato

Plasmid

#67699

Purpose

3' entry clone with 6xmyc nuclear localized tdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

tdTomato

Use

Tags

2A-6xMyc-NLS

Expression

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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R777-E084 Hs.FGFR1-nostop

Plasmid

#70368

Purpose

Gateway ORF clone of human FGFR1 [NM_001174063.1] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

FGFR1 (FGFR1 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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c7_HLL

Plasmid

#63125

Purpose

cysteine free Mm-cpn mutant

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_HLL (NEWENTRY )

Use

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pGD431

Plasmid

#132382

Purpose

contains the sequence of mRuby3for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP11

Plasmid

#80666

Purpose

Encodes the synthetic coiled coil peptide SYNZIP11

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP11

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133D

Plasmid

#69289

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLG169

Plasmid

#124367

Purpose

Xylose inducible system with the CRE site for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pxyl

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGD250

Plasmid

#132371

Purpose

contains the sequence of mClover3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mClover3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP20

Plasmid

#80674

Purpose

Encodes the synthetic coiled coil peptide SYNZIP20

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP20

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP17

Plasmid

#80671

Purpose

Encodes the synthetic coiled coil peptide SYNZIP17

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP17

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCBLggPro

Plasmid

#199736

Purpose

Golden Gate entry vector for promoter cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLggCDS

Plasmid

#199737

Purpose

Golden Gate entry vector for gene of interest cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLggTerm

Plasmid

#199738

Purpose

Golden Gate entry vector for terminator cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGD251

Plasmid

#132372

Purpose

contains the sequence of mCerulean3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mCerulean3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP21

Plasmid

#80675

Purpose

Encodes the synthetic coiled coil peptide SYNZIP21

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP21

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP18

Plasmid

#80672

Purpose

Encodes the synthetic coiled coil peptide SYNZIP18

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP18

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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