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Addgene

pGGEV_3_FRT_+1_OA-
(Plasmid #49311)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49311 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRT forward
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGEV_3_FRT_+1_OA- was a gift from Joachim Wittbrodt (Addgene plasmid # 49311 ; http://n2t.net/addgene:49311 ; RRID:Addgene_49311)
  • For your References section:

    Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. Kirchmaier S, Lust K, Wittbrodt J. PLoS One. 2013 Oct 7;8(10):e76117. doi: 10.1371/journal.pone.0076117. 10.1371/journal.pone.0076117 PubMed 24116091