Addgene

We narrowed to 15,233 results for: LEA

Showing: 4721 - 4740 of 15233 results

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11

Plasmid

#179681

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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SQT1659

Plasmid

#78743

Purpose

Human expression plasmid for AsCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Dlx-FLEX-hM4Di-mCherry

Plasmid

#209701

Purpose

Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

hM4D(Gi)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pYJ34-PB-CRISPR-BANCR-E1-gRNA

Plasmid

#131077

Purpose

long non-coding RNA BANCR knock out

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Insert

BANCR Exon1 gRNA (BANCR Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-Cre

Plasmid

#167939

Purpose

human CD81-TEV cleavage site-Cre

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Use

Tags

Cre recombinase and TEV protease cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-K45Q-GFP

Plasmid

#208777

Purpose

MSCV vector expressing K45Q mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

Change Lys 45 to Gln (K45Q)

Promoter

Available since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
p2T-CAG-REV1-p2A-GFP-PuroR

Plasmid

#153001

Purpose

Overexpression of human REV1

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

REV1 (REV1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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R26-H2B-mCherry HR donor vector

Plasmid

#137928

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-mCherry-bpA with homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_LuxSit_i_mTagBFP2

Plasmid

#194300

Purpose

Mammalian expression of the de novo luciferase, LuxSit-i

Depositor

Andy Yeh, David Baker

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Article

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Use

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-his-HYB

Plasmid

#64333

Purpose

his3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of his3 deletion gRNA cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
ND4.2-Left TALE-G1397-N-dead-mCherry

Plasmid

#179683

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

E1347A

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pNSA-ARS-CRISPR-BlastR

Plasmid

#101009

Purpose

Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pACS142

Plasmid

#204598

Purpose

Histone H3.3K9M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human H3.3 K9M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K9M - V5

Expression

Mammalian

Mutation

H3.3 K9M

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTMEM-PhoCl-Insert

Plasmid

#219674

Purpose

Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

TMEM-PhoCl-rtTA3

Use

Tags

PhoCl

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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