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Plasmid#118883Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-DARPinOFF7
Plasmid#182399PurposeBacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusionDepositorInsertVNp15-DARPinOFF7
UseTagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-LahT150
Plasmid#166785PurposeCleavage of double glycine motif in peptides to remove the leader sequenceDepositorInsertLahT150
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Bla(HA–RILP-FLAG)
Plasmid#102424PurposeDual tagged RILP for expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterTet-ResponsiveAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNAKv10.1mVenus
Plasmid#85705PurposeKv10.1 fused mVenusDepositorInsertKCNH1 (KCNH1 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_PPVs
Plasmid#119208PurposeCircularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with PPVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMutationPromoterCMVAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRE-SEAP
Plasmid#210511PurposeSEAP reporter vector for LAUNCHER systemDepositorInsertsecreted alkaline phosphatase
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLS.416
Plasmid#187207PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2)
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CN-B
Plasmid#196477PurposeProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCNDepositorInsertB-CN-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-forward
Plasmid#22073DepositorInsertPITX3 (ASMD Human)
UseCre/Lox; Zfn donorTagseGFPExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL1316
Plasmid#217958PurposeExpression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-mNeongreen_CydAB-mCherry
Plasmid#182391PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
cydAB-mCherry
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBAD-yhjH
Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
UseTagsExpressionBacterialMutationA52VPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
UseTagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
mito-yHS1
Plasmid#159153PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1
UseTagsCOX4 pre-sequenceExpressionYeastMutationPromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52545PurposeTargeting vector for homologous recombination in mouse ES cells; Hipp11 locusDepositorInsertsPhiC31o attP (full length)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFloat.T7_v1
Plasmid#133070PurposeEntry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-His6 and His6-3CExpressionBacterialMutationPromoterT7Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorInsertSmpd4 (Smpd4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only