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Showing: 4641 - 4660 of 154106 results

  3. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

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  5. AAV-PR rep/cap

    Plasmid
    #197565
    Purpose
    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
    Depositor
    Casey Maguire
    Article
    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089
    Inserts
    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    Use
    AAV
    Tags
    Expression
    Mutation
    contains 7mer insert PRPPSTH between amino acids …
    Promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197565-plasmid-map-sequence-id-388046

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  6. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid
    #106309
    Purpose
    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.
    Depositor
    James Zhe Liu
    Article
    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p
    Insert
    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    Use
    Tags
    6xflag-3xHaloTag(MtoL)
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106309-plasmid-map-sequence-id-204684

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  7. pMSP1E2D1

    Plasmid
    #161606
    Purpose
    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E2D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161606-plasmid-map-sequence-id-318420

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  8. pSBtet-GH

    Plasmid
    #60498
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60498-plasmid-map-sequence-id-97549

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  11. TFORF2241

    Plasmid
    #141985
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA3 (GATA3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141985-plasmid-map-sequence-id-391434

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  19. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid
    #223505
    Purpose
    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223505-plasmid-map-sequence-id-443299

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Showing: 4641 - 4660 of 154106 results