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pTRE3G-Bi-gePSI-pCamk2a-AcGFP

Plasmid

#133732

Purpose

Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

gePSI-β-chain
gePSI-α-chain
AcGFP1

Use

AAV

Tags

murine ornithine decarboxylase - degron (ODC36)

Expression

Mammalian

Mutation

Promoter

pCamk2a and pTRE3G-Bi

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSC-NF8-d1-eGFP

Plasmid

#122036

Purpose

AAV vector, NFkappaB-dependent eGFP expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al PLoS One. 2013;8(1):e53156. doi: 10.1371

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2155

Plasmid

#141065

Purpose

AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

Insert

SYN1-EmGFP-WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

April 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GEARBOCS

Plasmid

#218181

Purpose

CRISPR tool to target astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Vamp3-pHluorin

Plasmid

#190152

Purpose

Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp3 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

Plasmid

#111568

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Has Service

AAV5

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2284

Plasmid

#158419

Purpose

AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple349 (PDE6H Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple349

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D hM4D mCherry SV40

Plasmid

#92286

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

hM4D (CHRM4 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

GfaABC1D

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D hM3D mCherry SV40

Plasmid

#92284

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

hM3D (CHRM3 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

GfaABC1D

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
pSaCas9-1xms2-2x3’UTR

Plasmid

#122946

Purpose

For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-MS2-HBB 3' UTR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2214

Plasmid

#158417

Purpose

AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple265 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple265

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2022

Plasmid

#49129

Purpose

AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-PRS2/3:Con-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

DBH

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2021

Plasmid

#49128

Purpose

AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple302-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

DCX

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1 SV40-CMV-SaCas9-3xNLS

Plasmid

#78601

Purpose

AAV vector containing SaCas9

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

SV40-CMV-SaCas9-3xNLS

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

SV40, CMV promoters

Available since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only
pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple341

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSaCas9-1xPP7-2x3'UTR

Plasmid

#122947

Purpose

For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-PP7-HBB 3' UTR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

r1045-gggs-r1122 deletion

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1995

Plasmid

#49125

Purpose

AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple94-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GPR88

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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