Addgene

We narrowed to 9,017 results for: yeast

Showing: 4601 - 4620 of 9017 results

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Efg1-ΔDBD-Q443P

Plasmid

#216401

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Glutamine 443 to Proline, and deleted ami…

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Article

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Tags

Expression

Yeast

Mutation

Promoter

yeast CYC-GAL and T7 promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN100

Plasmid

#205073

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN380

Plasmid

#205076

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 380 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN296

Plasmid

#205075

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 296 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYP-ptef1-GFP

Plasmid

#199990

Purpose

Yeast/E coli shuttle vector expressing GFP from tef1 promoter

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3

Plasmid

#201413

Purpose

Bacterial protein expression of Edc3

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1

Plasmid

#201414

Purpose

Bacterial protein expression of Pat1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2

Plasmid

#201411

Purpose

Bacterial protein expression of Dcp2

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp1

Plasmid

#201410

Purpose

Bacterial protein expression of Dcp1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO7-6SIM

Plasmid

#190250

Purpose

Expression SUMO7-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

7SUMO-6SIM

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRS304-PTDH3-HEXAMER

Plasmid

#190251

Purpose

Expression hexamer component in yeast

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

hexamer

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Use

Tags

mCherry- AID

Expression

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Laura Davis, Ken Belanger

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Laura Davis, Ken Belanger

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-AUR1-msGFP2

Plasmid

#177101

Purpose

Expresses Aur1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

AUR1 (AUR1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-RBD2-6xmGFP

Plasmid

#177109

Purpose

Expresses Rbd2-6xmGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

RBD2 (RBD2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC2-msGFPx3

Plasmid

#177110

Purpose

Expresses Sec2-msGFPx3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SEC2 (SEC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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