-
Plasmid#62654PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expressionDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP73_sgRNA-1
Plasmid#88855PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_gRNA-Puro
Plasmid#84752PurposeExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPlatTET-gRNA2
Plasmid#82559PurposeAll in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.DepositorInsertdCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX_sgRNA_entry (CJT32)
Plasmid#181782PurposeExpresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-1
Plasmid#66202Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU3b
Plasmid#66198Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAtU3bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_sgRNA-ver2
Plasmid#126776PurposegRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNADepositorInsertsgRNA (for SpCas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-29
Plasmid#66203Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-gRNA-Puro
Plasmid#121121PurposeExpression of a gRNA together with a Puromycin resistanceDepositorInsertgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-spsgRNA2
Plasmid#64114PurposeVector for expression of Sp sgRNA2 in mammalian cellsDepositorInsertSp sgRNA2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA
Plasmid#138271PurposeDual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)DepositorInsertsgBG, sgTS
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6b
Plasmid#66196Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU6bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6a
Plasmid#66194Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU6aAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only