Addgene

We narrowed to 472 results for: PA-GFP

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plIG-128_HA-GD2-28z_CAR_TFxTF_Library

Pooled Library

#207480

Purpose

HA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-106_NY-ESO-1_TCR_SR_Library

Pooled Library

#207474

Purpose

NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-107_NY-ESO-1_TCR_TF_Library

Pooled Library

#207475

Purpose

NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-109_CD19-BBz_CAR_SR_Library

Pooled Library

#207476

Purpose

CD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-110_CD19-BBz_CAR_TF_Library

Pooled Library

#207477

Purpose

CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-124_HA-GD2-28z_CAR_SR_Library

Pooled Library

#207479

Purpose

HA-GD2-28z CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cel

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-132_CD19-28z_CAR_TF_and_SR_Library

Pooled Library

#207481

Purpose

CD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knoc

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
Ma-pREP

Plasmid

#197571

Purpose

Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

Chloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)

Use

Tags

Expression

Bacterial

Mutation

111TAG and 8TAG/112TAG

Promoter

AraC, T7, cat promoter (constitutive), and lpp (c…

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-HITI

Plasmid

#87117

Purpose

AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPAS-E

Plasmid

#39867

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein PAS domain

Use

Tags

E coil

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT sfGPF 150TAG

Plasmid

#154766

Purpose

plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in GFP reporter, U25C in PylT

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRosa26-TG

Plasmid

#40026

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides 1-274 and insertion of FRT, …

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mutation

TVA mammalian codon optimized

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pRosa26-GT

Plasmid

#40025

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

Plasmid Kit

#1000000087

Purpose

Tol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disorders

Depositor

Nicholas Cole

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2019

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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