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Addgene
Showing: 441 - 460 of 717 results

  1. AAV-∆Hapln1

    Plasmid
    #200778
    Purpose
    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter
    Depositor
    Alexander Dityatev
    Article
    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d
    Insert
    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    Use
    AAV
    Tags
    cfGFP
    Expression
    Mutation
    deleted lectican binding domain (aminoacids 40-15…
    Promoter
    Synapsin
    Availability
    Academic Institutions and Nonprofits only
    200778-plasmid-map-sequence-id-394043

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  4. pαH-S-GSAS-deltaH69/V70

    Plasmid
    #171744
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171744-plasmid-map-sequence-id-337363

    Enlarge

  5. pαH-S-GSAS-D614G-Y453F

    Plasmid
    #171745
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-Y453F variant
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-Y453F variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171745-plasmid-map-sequence-id-337717

    Enlarge

  6. pαH-S-GSAS-D614G-I692V

    Plasmid
    #171746
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171746-plasmid-map-sequence-id-337719

    Enlarge

  7. pαH-S-GSAS-D614G-N501Y

    Plasmid
    #171748
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variant
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171748-plasmid-map-sequence-id-337770

    Enlarge

  8. pαH-S-GSAS-D614G-E484K

    Plasmid
    #171751
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-E484K variant
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-E484K variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171751-plasmid-map-sequence-id-337763

    Enlarge

  9. pαH-S-GSAS-D614G-K417N

    Plasmid
    #171752
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N variant
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-K417N variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171752-plasmid-map-sequence-id-337762

    Enlarge

  10. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  11. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  12. pαH-S-GSAS-B.1.351.v3

    Plasmid
    #173787
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 3
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    173787-plasmid-map-sequence-id-340760

    Enlarge

  13. pαH-S-GSAS-B.1.351.v4

    Plasmid
    #173788
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    173788-plasmid-map-sequence-id-352110

    Enlarge

  14. pαH-S-GSAS-P1-like

    Plasmid
    #173789
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    173789-plasmid-map-sequence-id-340758

    Enlarge

  15. pαH-S-GSAS-B.1.351.v5

    Plasmid
    #173790
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 5
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    173790-plasmid-map-sequence-id-340761

    Enlarge

  16. pαH-S-GSAS-B.1.351.v6

    Plasmid
    #173791
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 6
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    173791-plasmid-map-sequence-id-339984

    Enlarge

Showing: 441 - 460 of 717 results