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We narrowed to 14,966 results for: grna

Showing: 4561 - 4580 of 14966 results

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Benjamin Kleinstiver, Keith Joung

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro

Plasmid

#167911

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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hU6-pegRNA-EF-1α-Cre Template

Plasmid

#196036

Purpose

The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6 and EF-1-alpha

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Use

Tags

Expression

Mammalian

Mutation

Promoter

NA

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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v1em-Cterm-PE2max-U6-pegRNA

Plasmid

#198733

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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v1em-Nterm-PE2max-U6-sgRNA

Plasmid

#198732

Purpose

AAV genome encoding N-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-eCas9-GFP-U6-gRNA

Plasmid

#79145

Purpose

All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

Depositor

Jizhong Zou

Insert

eSpCas9(1.1)

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE, modified BFP

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Article

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

PU6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE, modified BFP

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pUbi_dCas9-BFP-KRAB-gRNA

Plasmid

#221503

Purpose

CRISPRi machinery fish codon optimized expressing plasmid

Depositor

Alessandro Ori

Insert

dCas9-tagBFP-KRAB

Use

Fish expression

Tags

Expression

Mutation

D10A and H839A point mutations

Promoter

Zebrafish Ubiquitin promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Expression

Mutation

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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