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We narrowed to 14,722 results for: LEA

Showing: 4561 - 4580 of 14722 results

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pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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R26-EGFP HMEJ donor vector

Plasmid

#137927

Purpose

ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro_shTBK1_3185

Plasmid

#167294

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA TBK1 (TBK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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R26-H2B-mCherry HR donor vector

Plasmid

#137928

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-mCherry-bpA with homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-tTA

Plasmid

#162598

Purpose

human CD81-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Use

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

Plasmid

#129708

Purpose

Express cre-dependent KASH-EGFP and scramble shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSBK.079

Plasmid

#187218

Purpose

Expresses Eco1 RT and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 Reverse Transcriptase
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23115 and T7/lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-REV1-p2A-GFP-PuroR

Plasmid

#153001

Purpose

Overexpression of human REV1

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

REV1 (REV1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_LuxSit_i_mTagBFP2

Plasmid

#194300

Purpose

Mammalian expression of the de novo luciferase, LuxSit-i

Depositor

Andy Yeh, David Baker

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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R26-H2B-EGFP HR donor vector

Plasmid

#137925

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-EGFP-bpA with homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-21

Plasmid

#172749

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-21

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-37

Plasmid

#172756

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-37

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh and hU6

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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MARIO/ pcDNA3

Plasmid

#124838

Purpose

mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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