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Plasmid#32446PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)DepositorArticleInsertG-GECO1.2
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP720-N1
Plasmid#136560PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720DepositorInsertmiRFP720
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
UseTagsFis1ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
myc-Raptor
Plasmid#1859DepositorInsertRaptor (RPTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 T210D (Nigg LA183)
Plasmid#41158DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationT210DPromoterCMVAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 K82R (Nigg LA184)
Plasmid#41157DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationK82RPromoterCMVAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFAM-SNAPf
Plasmid#187909PurposeMammalian cell expression of SNAPf-labeled TFAMDepositorInsertTFAM (TFAM Human)
UseTagsSNAPfExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP680-tubulin
Plasmid#136575PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP680DepositorInsertmiRFP680-tubulin
UseTagsmiRFP680ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-MitoTimer
Plasmid#50547PurposeExpresses the Timer protein targeted to the mitochondria under a Tet-inducible promoterDepositorInsertMitoTimer
UseTagsExpressionMammalianMutationF12S mutation in TimerPromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-EGFP-CNA35
Plasmid#61603Purposebacterial expression of N-terminal fusion protein of CNA35 with EGFPDepositorInsertEGFP and CNA35
UseTagsHis tagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-clathrin
Plasmid#136563PurposeClathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703-clathrin
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybGam-WPRE
Plasmid#207662PurposeControl for CybSEP2DepositorInsertGamillus
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted gamill…PromoterhSynapsinAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only