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Plasmid#123347PurposeExpression of human SHIP1DepositorInsertINPP5D (INPP5D Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDC-DIO-GFP-shRNA.scramble
Plasmid#184331PurposeExpresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expressionDepositorInserthdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiTagsExpressionMammalianMutationPromoterhistidine decarboxylase promoter fragment that g…Available sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-SpdCas9-W3SL
Plasmid#210708PurposeThis Plasmid express hSyn promoter driven SpdCas9DepositorInsertS. Pyogenes dCas9
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationD10A/H840APromoterhSynAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD9
Plasmid#195847PurposeExpresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556HDepositorInsertFZD9 (FZD9 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MRP1-CD4d3+4-bio
Plasmid#73120PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding MRP1 with rat CD4d3+4-bioDepositorInsertMRP1 (ABCC1 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCP-3x9aaTAD
Plasmid#210707PurposeThis Plasmid express EF1a promoter driven MCP-3x 9aa transactivation moduleDepositorInsertMCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Tev_ACTA1 (alpha-actin)
Plasmid#188452PurposeExpress in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.DepositorInsertACTA1 (ACTA1 Human)
UseTags6xHis-FLAGExpressionMammalianMutationWTPromoterCMVAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 28A
Plasmid#53447PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationI914T mutation; All 28 SQ/TQ motifs in N-term of …PromoterAvailable sinceDec. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
W118-1_Flag-hIRAK1
Plasmid#180405Purposelentiviral transduction of human IRAK1 (with an N-terminal Flag tag) geneDepositorInsertIRAK1 (IRAK1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fer-SH2
Plasmid#46425DepositorInsertFer (FER Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-558PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
GlycophorinC-CD4d3+4-bio
Plasmid#73096PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinC with rat CD4d3+4-bioDepositorInsertGlycophorin C (GYPC Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184V
Plasmid#106175PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterhSynapsinAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgA1
Plasmid#187009PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_Cre
Plasmid#201198PurposeAAV vector for cre expression under EF1 promoterDepositorInsertscre
EF1alpha promoter
UseAAVTagsExpressionBacterial and MammalianMutationnuclear targeting crePromoterN/A and hEF1alpha promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V
Plasmid#119723PurposeAAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertsCaMPARI2
CaMPARI2
UseAAVTagsNES-hisExpressionMammalianMutationF391W, L398VPromoterhSyn1Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPI-LUC7L2-3xFLAG
Plasmid#201641PurposeOver-expression of 3xFLAG-tagged human LUC7L2DepositorInsertLUC7L2 (LUC7L2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-GFP-shRNA-GluN1
Plasmid#182502PurposeExpresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoterDepositorInsertpan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
UseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterfragment of histidine decarboxylase gene promoter…Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1 C121S
Plasmid#53197PurposeExpresses HA tagged human MEK1 C121S from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationC121SPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Duffy-CD4d3+4-bio
Plasmid#73122PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Duffy with rat CD4d3+4-bioDepositorInsertDuffy (ACKR1 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only