Human SHIP1 (clone hp51CN)
(Plasmid
#123347)
-
PurposeExpression of human SHIP1
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 123347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 9450
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameINPP5D
-
Alt nameSHIP
-
Alt nameSHIP1
-
Alt namehp51CN; p150Ship; SHIP; SHIP-1; SHIP1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)4000
-
GenBank IDNM_005541 EMBL sequence data base X98429
-
Entrez GeneINPP5D (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer M13 forward primer
- 3′ sequencing primer M13 reverse primer (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digestion of this plasmid with EcoRI and XhoI should yield three bands of 1.75, 2.1 and 5.6 kb
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Human SHIP1 (clone hp51CN) was a gift from Christophe Erneux (Addgene plasmid # 123347 ; http://n2t.net/addgene:123347 ; RRID:Addgene_123347) -
For your References section:
Cloning and expression of a human placenta inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase. Drayer AL, Pesesse X, De Smedt F, Woscholski R, Parker P, Erneux C. Biochem Biophys Res Commun. 1996 Aug 5;225(1):243-9. doi: 10.1006/bbrc.1996.1161. 10.1006/bbrc.1996.1161 PubMed 8769125