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pBbdCas9S_P0526-sgRNA500
Plasmid#149641Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)
Plasmid#149574Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)
Plasmid#149581Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Hy_pMT eGFP-RVFV NSs HhRz
Plasmid#91801PurposeExpresses a non-polyadenylated, translatable EGFP mRNA in DrosophilaDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4(RLR)-Bre1
Plasmid#116884PurposeFLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutationsDepositorInsertLINXa4(RLR)-Bre1
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11A, LINXa3 wi…PromoterADH1Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRFP shRNA-resistant TrkB WT
Plasmid#72714PurposeMammalian expression of TrkB fused to RFP in C-terminus, shRNA resistantDepositorInsertTrkB
UseTagsRFPExpressionMammalianMutationshRNA-resistant TrkB (T300C, T301A, C302G, C306A,…PromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA PTEN KD2
Plasmid#162009PurposePTEN shRNADepositorInsertPTEN (PTEN Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM55361
Plasmid#153227PurposeControl plasmid for knockout of AP3 geneDepositorInsertCas9, guide RNA for TRY and CPC and FAST marker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Pax7bait-T2a-Cherry-pA
Plasmid#111154Purposeknock in bait for Axolotl Pax7 locusDepositorInsertPax7bait-T2a-Cherry-pA
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-C-8His-Flag
Plasmid#157678PurposeC-terminal tagging vectorDepositorInsert8His-Flag
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXA9 (murine) HIS-tag pET
Plasmid#8492DepositorInsertHoxA9 (Hoxa9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationBamHI/BalI fusion into amino acid #2 of HOXA9; ma…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT7 LDIG69AAAA
Plasmid#61560PurposeHs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutantDepositorInsertPRMT7 (PRMT7 Human)
UseTagsGSTExpressionBacterialMutationLDIG 69-72 AAAAPromoterAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA053
Plasmid#128515PurposeExpresses SFA8 in PichiaDepositorInsertSFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)
UseTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPTXLUC
Plasmid#68504Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutationPromoterOPTXAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-PCYC
Plasmid#60335PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbdCas9S_Psyn-sgRNAflaB
Plasmid#149642Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAmreB
Plasmid#149653Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only
