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Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-AkCas12b
Plasmid#121950PurposeE. coli expression, protein purificationDepositorInsertAkCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_NES_C1
Plasmid#85061PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tagDepositorInsertS-Luc_CLIP
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HybridBarcode
Plasmid#174037PurposeFor Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sitesDepositorInsertCRISPR target sites
UseTol2 transgenesisTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgIGR_dual-MTS-3x-Set I
Plasmid#162764PurposeSeparately expressing 3 unique sgIGR_dual-MTS (SetI)DepositorInsertsgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141C2.0
Plasmid#99905PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Trip-GFP-hOCLN-ΔN
Plasmid#32792DepositorInsertOCLN (OCLN Human)
UseLentiviralTagsGFPExpressionMammalianMutationDeletion of aa 1-63PromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AagRNA
Plasmid#121953PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b tracrRNA/crRNA duplex
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p27(kip1)
Plasmid#50026PurposepENTR vector for human cyclin-dependent kinase inhibitor 1BDepositorInsertp27(kip1) (CDKN1B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-TALE-mCherry-15b
Plasmid#138287PurposeExpress tTALE-mCherryDepositorInsert7-mer tTALE-mCherry
UseTALENTags6x His-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Trip-GFP-hOCLN-ΔEL1
Plasmid#32796DepositorInsertOCLN (OCLN Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of aa 94-128PromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mEGFP/FRT/TO
Plasmid#27762DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/SPTBN1-FLT3
Plasmid#168115PurposeMammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemiaDepositorInsertSPTBN1-FLT3
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-link/link-C
Plasmid#61965PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)DepositorInsertsN-mCherry-link
link-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_2FLAG-AKAP79ΔCN
Plasmid#183451PurposeExpression of human AKAP79 lacking the calcineurin binding site (Δ337-343)DepositorInsertAKAP5 (AKAP5 Human)
UseTags2xFLAGExpressionMammalianMutationaa337-343 deletedPromoterCMVAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only