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Showing: 4461 - 4480 of 5522 results

  1. pLCHKO_Ptbp_ex8_2

    Plasmid
    #155082
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155082-plasmid-map-sequence-id-308510

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  5. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

    Plasmid
    #105809
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    FLAG-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105809-plasmid-map-sequence-id-203432

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  19. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87421-plasmid-map-sequence-id-441601

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  20. Ma-Barnase

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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Showing: 4461 - 4480 of 5522 results