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Plasmid#131791PurposeLentiviral vector expressing WT TBK1DepositorInsertTBK1 WT (TBK1 Human)
UseLentiviralTagsN-FLAG-HAExpressionMammalianMutationWT full lengthPromoterCMVAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPExpressionMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8m
Plasmid#169248PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.DepositorInsertRiboL1-jGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1_ICD
Plasmid#121480Purposeexpression of human PD-L1 in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseTagsEGFPExpressionMammalianMutationTM+ICDPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1_ECD
Plasmid#121479Purposeexpression of human PD-L1 in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseTagsEGFPExpressionMammalianMutationECD+TMPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
Plasmid#134859PurposeExpresses EGFP-tagged ERGIC markerDepositorInsertERGIC53 (Lman1 Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA2 (SMARCA2 Human)
UseTagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB3
Plasmid#120288PurposeProduce human recombinant TGFB3 in DE3 cellsDepositorInsertTransforming Growth Factor-β3 (TGFB3 Human, Synthetic)
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TagBFP-hCoilin
Plasmid#182589Purposeexpresses bfp-hcoilin in mammalian cellsDepositorInsertCoilin (COIL Human)
UseLentiviralTagsBFPExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-cMYC/pLVX-Puromycin
Plasmid#180278PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).DepositorInsertZsGreen1-cMYC fusion protein MYC (MYC Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM33
Plasmid#65398PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM33 (TRIM33 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAG104 FAST
Plasmid#130720PurposeExpresses FAST (also called YFAST) in mammalian cellsDepositorInsertFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8s
Plasmid#167572PurposePlease see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.DepositorInsertRibo-GCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATG-3925 (CBG2)
Plasmid#167839PurposeExpresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cellsDepositorInsertClick Beetle Green 2
UseTags6-HisExpressionBacterial and MammalianMutationQ348H. Click Beetle Green 2 (CBR2) has 3 amino a…PromoterCMV, T7Available sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-CAG-RLuc
Plasmid#83280Purposerecombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoterDepositorInsertRenilla Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP1-Flag-His-7xKQ
Plasmid#111573PurposeExpresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cellsDepositorInsertPARP1 (PARP1 Human)
UseTagsC-terminal 6xHis and C-terminal FlagExpressionInsectMutationlysine to glutamine mutation at the following ami…Promoterpolyhedrin promoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-XBP1 mNeonGreen NLS
Plasmid#115971PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)DepositorInsertX-box binding protein 1 (XBP1 Human, Synthetic)
UseRetroviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only