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Plasmid#196877PurposeNeuron-specific expression of mOrange2 reporterDepositorInsertmOrange2
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-td-mCherry-CI
Plasmid#196851PurposeExpression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoterDepositorInserttd-mCherry
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2
Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG
Plasmid#185347PurposeMembrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tagDepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
KTK_139
Plasmid#180565PurposeSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2DepositorInsertSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin-S796A
Plasmid#186434PurposeExpresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
UseTagsmCherryExpressionMammalianMutationChanged Serine 796 to AlaninePromoterCMV IE94Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_113
Plasmid#180540PurposeEntry vector containing Signal peptide region of CsgA including SecB-N22 sequencedDepositorInsertSignal peptide region of CsgA including SecB-N22 sequenced
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT K78E-IRES-Puro
Plasmid#182591Purposeexpresses TERT K78E in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationK78EPromoterEF-1aAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_095
Plasmid#180524PurposedCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assemblyDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_324
Plasmid#180566PurposeSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2aDepositorInsertSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
iDianiSnFR
Plasmid#177741PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiDianiSnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG660 lyn11-pFAST
Plasmid#172863Purposeexpresses lyn11-pFAST in mammalian cells (inner membrane labeling)DepositorInsertLyn11-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2
Plasmid#175751PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A
Plasmid#175752PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50ADepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationY49A L50APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
iCytSnFR
Plasmid#177738PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCytSnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_BrEt_SnFR
Plasmid#177740PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_BrEt_SnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_F_SnFR
Plasmid#177739PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_F_SnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TTBG
Plasmid#115232PurposeLentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2DepositorInsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only