pCS2-mCherry-Cenexin-S796A
(Plasmid #186434)

• Purpose: Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos
• Depositing Lab: Heidi Hehnly
• Publication: Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. doi: 10.1091/mbc.E22-01-0015. Epub 2022 May 24.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4091
• Total vector size (bp): 7935
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cenexin
• Alt name: ODF2
• Species: H. sapiens (human)
• Insert Size (bp): 3844
• Mutation: Changed Serine 796 to Alanine
• GenBank ID: NM_153435.1
• Entrez Gene: ODF2 (a.k.a. CT134, ODF2/1, ODF2/2, ODF84)
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kyung S Lee Lab of the National Cancer Institute and Stephen Doxsey Lab University of Massachusetts Medical School Program in Molecular Medicine

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Published in: Soung, N.-K., Kang, Y. H., Kim, K., Kamijo, K., Yoon, H., Seong, Y.-S., Kuo, Y.-L., Miki, T., Kim, S. R., Kuriyama, R., Giam, C.-Z., Ahn, C. H., & Lee, K. S. (2006). Requirement of hCenexin for Proper Mitotic Functions of Polo-Like Kinase 1 at the Centrosomes. Molecular and Cellular Biology, 26(22), 8316–8335. https://doi.org/10.1128/mcb.00671-06

Hung HF, Hehnly H, Doxsey S. The Mother Centriole Appendage Protein Cenexin Modulates Lumen Formation through Spindle Orientation. Curr Biol. 2016 Mar 21;26(6):793-801. doi: 10.1016/j.cub.2016.01.025. Epub 2016 Mar 3. PMID: 26948879; PMCID: PMC5027652.

Please visit https://www.biorxiv.org/content/10.1101/2022.01.09.475500v2 for bioRxiv preprint.