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Plasmid#122845PurposeN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only
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GST-mCh-FYVE
Plasmid#192289PurposeExpresses GST- and mCherry-tagged HRS FYVE in e ColiDepositorInsertHRS FYVE domain
UseTagsGST-TEV-mCherryExpressionBacterialMutationPromotertacAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP GFP-NBR1
Plasmid#38283DepositorInsertNext to BRCA1 gene 1 protein (NBR1 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-Tandem-mCherry-EGFP
Plasmid#123216PurposeN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 L343A
Plasmid#38279DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged L343APromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
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GST-TAX1BP1
Plasmid#171423PurposeFor the purifcation of TAX1BP1 proteinDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHmK-C1
Plasmid#87866PurposeBackbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1DepositorInsertpHluorin-mKate2 fusion
UseTagspHluorin-mKate2 fusionExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 K7A, D69A
Plasmid#38281DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged K7A, D69APromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 D337, 338, 339A
Plasmid#38280DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged D337A, D338A, D339APromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 dC
Plasmid#38282DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationTruncate mutant 1-265 amino acids residuesPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
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GST-TEVcs-FIP200-MBP
Plasmid#171410PurposeFor the purifcation of human ULK1 complexDepositorInsertFIP200 (RB1CC1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP-SNX9
Plasmid#170551PurposeTo monitor the turnover of SNX9 in lysosomesDepositorInsertSNX9 (SNX9 Human)
UseRetroviralTagsSNX9 in tandem with mCherry and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
WIPI2d-TEVcs-TSF
Plasmid#171419PurposeFor the purifcation of WIPI2d proteinDepositorInsertWIPI2d (WIPI2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP WT
Plasmid#123097PurposeExpresses 3xFLAG-GABARAP wild-type in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-OPTN(F178A)
Plasmid#119681PurposeFor mammalian expression of LIR mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationF178APromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-OPTN(D474N)
Plasmid#119683PurposeFor mammalian expression of UBD mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsmCherryExpressionMammalianMutationD474NPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-TAX1BP1(ΔUBD)
Plasmid#119689PurposeFor mammalian expression of TAX1BP1 without its UBDDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationUBD deletion (1-755)PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only