Addgene

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Showing: 4301 - 4320 of 16985 results

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pACE-K2P10.1-StrepTagII

Plasmid

#191475

Purpose

Expresses K2P10.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P10.1 (KCNK10, TREK-2 Human)

Use

Tags

Expression

Insect

Mutation

N144Q (uniprot P57789-1)

Promoter

polH

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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AID-mTagBFP2-loxP_myo2_neoR-loxP

Plasmid

#194055

Purpose

Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Use

Tags

codon optimized mTagBFP2

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pES002 MBP-GSK3β_S9A-HA-His

Plasmid

#196184

Purpose

Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Insert

GSK3B (GSK3B Human)

Use

Tags

HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p7058 pHAGE-P-CMVt-N-HA-GAW-FDFT1

Plasmid

#100151

Purpose

Expresses FDFT1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FDFT1 (FDFT1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p7129 pHAGE-P-CMVt-N-HA-GAW-HM13

Plasmid

#100155

Purpose

Expresses HM13

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

NM_030789.3 (HM13 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAL119-Flt3L

Plasmid

#21910

Depositor

Maria Castro

Article

Ali et al Mol Ther. 2004 Dec . 10(6):1071-84.

Insert

Human Soluble Fms-like Tyrosine Kinase 3 Ligand (FLT3LG Human)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

Plasmid

#167295

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAK (BAK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

Plasmid

#158953

Purpose

Express human RAD51(T309A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed threonine 309 to alanine

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3B-copGFP-Puro

Plasmid

#107342

Purpose

Expresses UBE3B fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

Ubiquitin-protein ligase E3B (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+8 Flag3HuR

Plasmid

#135950

Purpose

Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Tags

Flag3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p45)

Plasmid

#135947

Purpose

Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3B(C1036A)-copGFP-Puro

Plasmid

#107344

Purpose

Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A)copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

mutation C1036A

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-GFP-EBA175-Flag

Plasmid

#154970

Purpose

Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

insert codes for EBA175 residues 1284 to 1462

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p7130 pHAGE-P-CMVt-N-HA-GAW-KIF20A

Plasmid

#100156

Purpose

Expresses KIF20A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

KIF20A (KIF20A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-V0A

Plasmid

#210347

Purpose

Expresses ATP6V0A in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

V-ATPase V0A (ATP6V0A1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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p415 TEF Su9‐roGFP2‐Tsa2ΔCR

Plasmid

#83240

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

Su9-roGFP2-Tsa2dCr

Use

Tags

roGFP2 fusion with Tsa2dCr with Su9 targetting se…

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP(iL)-Grx1(human)

Plasmid

#65002

Purpose

Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1 (GLRX Human)

Use

Tags

roGFP(iL)

Expression

Yeast

Mutation

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS C396/7A-HA

Plasmid

#130917

Purpose

Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS C396/7A

Use

Tags

HA

Expression

Mammalian

Mutation

mutated residues 396 and 397 to alanines

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E

Plasmid

#100147

Purpose

Expresses EIF3E

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3E (EIF3E Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized

Plasmid

#172345

Purpose

Expression of FLAG-tagged, codon-optimized, full-length SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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