Skip to main content
Addgene
Showing: 4221 - 4240 of 5522 results

  2. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Article
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    84041-plasmid-map-sequence-id-164306

    Enlarge

  6. pCDF-Mm2

    Plasmid
    #197100
    Purpose
    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    PylRS variant, Pyl tRNA, kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197100-plasmid-map-sequence-id-393189

    Enlarge

  8. pHLmMBP-3

    Plasmid
    #72345
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72345-plasmid-map-sequence-id-133752

    Enlarge

  11. pHLmMBP-11

    Plasmid
    #72349
    Purpose
    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72349-plasmid-map-sequence-id-133760

    Enlarge

  15. pcDNA3.1-KozATG-dL5-2XG4S-mCer3

    Plasmid
    #73207
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    KozATG-dL5-2XG4S-mCer3
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73207-plasmid-map-sequence-id-139724

    Enlarge

Showing: 4221 - 4240 of 5522 results